Uncoated Human GDNF(Glial Cell Line Derived Neurotrophic Factor) ELISA Kit

$98.99 ~~$500.95~~

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Category : Life Science Products

Uncoated Human GDNF(Glial Cell Line Derived Neurotrophic Factor) ELISA Kit

Detection Range

0.31-20 ng/mL

Application

The new series of Elabscience Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

For in vitro quantitative determination of GDNF concentration in Human serum, plasma.

Properties

Assay type	Sandwich
Format	96T5
Assay time	24h
Reactivity	Human
Storage	-20. Please refer to the instructions for details.
Sample type	serum, plasma
Sample volume	100L

Kit components

Item	Specifications	Storage
Micro ELISA pre-Plate(Dismountable)	96T55 plates, 96T	-20, 12 months
Reference Standard	96T51 vial
Concentrated Capture Ab	96T51 vial 120L
Concentrated Biotinylated Detection Ab	96T51 vial 120L
Concentrated HRP Conjugate	96T51 vial 120L	-20(Protect from light), 12 months
Product Description	1 copy	/
Certificate of Analysis	1 copy

Test Principle

	1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.
	2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.
	3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.
	4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.
	5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.